Skip to main content
Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms

Fig. 1

Blocking IL-1 receptor with IL-1Ra normalizes both phosphorylation and membrane expression of the GluA1 and GluA2 subunits in hyperammonemic rats. IL-1Ra, an antagonist of IL-1 receptor, was added to hippocampal slices. Membrane expression of GluA1 (a) and GluA2 (b) subunits and phosphorylation of GluA1 at Ser831 (c) and of GluA2 at Ser880 (d) were analyzed as described in the “Methods” section. Values are expressed as percentage of basal levels in control rats and are the mean ± SEM of 24, 34, 31, and 18 rats per group in a, b, c, and d respectively. Data were analyzed by two-way ANOVA. In a, F (1, 92) = 0.004596 for effect of HA, p = 0.9461; F (1, 92) = 11.77 for effect of IL1Ra, p = 0.0009; and F (1, 92) = 0.0368 for interaction, p = 0.0368. In b, F (1, 132) = 4.893 for effect of HA, p = 0.0287; F (1, 132) = 41.17 for effect of IL1Ra, p < 0.0001; and F (1, 132) = 9.953 for interaction, p = 0.0020. In c, F (1, 118) = 1.122 for effect of HA, p = 0.2915; F (1, 118) = 11.24 for effect of IL1Ra, p < 0.0001; and F (1, 118) = 15.73 for interaction, p = 0.0001. In d, F (1, 68) = 1.028 for effect of HA, p = 0.3142; F (1, 68) = 4.783 for effect of IL1Ra, p = 0.0322; and F (1, 68) = 22.11 for interaction, p < 0.0001. Values significantly different from control rats are indicated by asterisk and from hyperammonemic rats are indicated by “a”. Bonferroni post-test: *p < 0.05, ***p < 0.001, aaap < 0.001

Back to article page