Fig. 2

IL-1 receptor-mediated activation of Src leads to the alterations in membrane expression and phosphorylation of GluA1 and GluA2 subunit in hyperammonemic rats. IL-1Ra or PP2, an inhibitor of Src kinase, were added to hippocampal slices. Phosphorylation of Src at Tyr416 (a), of GluA1 at Ser831 (c), of GluA2 at Ser880 (e), and membrane expression of GluA1 (b) and GluA2 (d) were analyzed as described in the “Methods” section. Values are expressed as percentage of basal levels in control rats and are the mean ± SEM of 24, 22, 33, 32, and 18 rats per group in a, b, c, d, and e respectively. Data were analyzed by two-way ANOVA. In a, F (1, 92) = 0.005730 for effect of HA, p = 0.9398; F (1, 92) = 5.434 for effect of IL1Ra, p = 0.0219; and F (1, 92) = 3.058 for interaction, p = 0.0837. In b, F (1, 83) = 1.361 for effect of HA, p = 0.2466; F (1, 83) = 9.148 for effect of PP2, p = 0.0033; and F (1, 83) = 8.418 for interaction, p = 0.0048. In c, F (1, 127) = 4.230 for effect of HA, p = 0.0418; F (1, 127) = 5.912 for effect of PP2, p = 0.0164; and F (1, 127) = 2.146 for interaction, p = 0.1454. In d, F (1, 123) = 1.545 for effect of HA, p = 0.2162; F (1, 123) = 8.038 for effect of PP2, p = 0.0054; and F (1, 123) = 11.17 for interaction, p = 0.0011. In e, F (1, 68) = 7.756 for effect of HA, p = 0.0069; F (1, 68) = 7.864 for effect of PP2, p = 0.0066; and F (1, 68) = 44.50 for interaction, p < 0.0001. Values significantly different from control rats are indicated by asterisk and from hyperammonemic rats are indicated by “a”. Bonferroni post-test: ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^ap < 0.05, ^aaap < 0.001