Fig. 3

IL-1 receptor and Src activation lead to increased phosphorylation at Tyr1472 and membrane expression of the GluN2B subunit in hyperammonemic rats. IL-1Ra or PP2, an inhibitor of Src kinase, were added to hippocampal slices. Phosphorylation of GluN2B at Tyr1472 (a, c) and membrane expression of GluN2B (b, d) were analyzed as described in the “Methods” section. Values are expressed as percentage of basal levels in control rats and are the mean ± SEM of 27, 37, 28, and 37 rats per group in a, b, c, and d respectively. Data were analyzed by two-way ANOVA. In a, F (1, 104) = 0.3170 for effect of HA, p = 0.5746; F (1, 104) = 5.592 for effect of IL-1Ra, p = 0.0199; and F (1, 104) = 9.208 for interaction, p = 0.0030. In b, F (1, 143) = 3.642 for effect of HA, p = 0.0583; F (1, 143) = 10.33 for effect of IL-1Ra, p = 0.0016; and F (1, 143) = 9.704 for interaction, p = 0.0022. In c, F (1, 106) = 1.727 for effect of HA, p = 0.1917; F (1, 106) = 6.991 for effect of PP2, p = 0.0094; and F (1, 106) = 1.457 for interaction, p = 0.2302. In d, F (1, 143) = 8.814 for effect of HA, p = 0.0035; F (1, 143) = 5.432 for effect of PP2, p = 0.0212; and F (1, 143) = 4.963 for interaction, p = 0.0275. Values significantly different from control rats are indicated by asterisk and from hyperammonemic rats are indicated by “a”. Bonferroni post-test: ^*p < 0.05, ^***p < 0.001, ^ap < 0.05, ^aap < 0.01, ^aaap < 0.001