Fig. 6
From: Ablation of caspase-1 protects against TBI-induced pyroptosis in vitro and in vivo
Neuron injury-induced inflammation and pyroptosis. Relative to the control group (cultured primary neuron without mechanical or chemical treatment), scratch, stretch, and LPS/ATP stimulation induced significant increases in pro-inflammatory cytokines: a IFN-γ, b IL-6, c IL-1β, and d IL-18. e mRNA expression of TLR4, MyD88, and NLRP3 after scratch, stretch, and LPS/ATP treatments. Expression of these transcripts increased by 4.5-fold, 4.0-fold, and 9.0-fold, respectively, compared to control neurons. f Caspase-1 activity was enhanced at 10.3-fold, 12.4-fold, and 21.6-fold following scratch, stretch, and LPS/ATP stimulation, compared to the control group. g Cell lysate and culture supernatant were collected; pyroptosis-related protein expression was detected by WB after scratch, stretch, and LPS/ATP stimulation in cultured primary neuron. Caspase-1 and its fragments (p10 and p20) leaked to the culture supernatant. The histogram was used to analyze protein expression in the cell lysate. Data are presented as the mean ± SEM. ***p < 0.001 versus normal group; one representative experiment of five was shown