Fig. 7
From: Ablation of caspase-1 protects against TBI-induced pyroptosis in vitro and in vivo
Caspase-1 deficiency attenuated neuron injury-induced apoptosis and pyroptosis. a Mechanical scratch, equiaxial stretch, and LPS/ATP stimulation induced apoptosis and caspase-1 knockdown with siRNA and pharmacologic inhibition with VX-765 reduced cellular apoptosis following scratch, stretch, and LPS/ATP. b Supernatant LDH concentration was detected when caspase-1 was a deficiency (siRNA and VX-765). c Caspase-1 activity was enhanced with scratch, stretch, and LPS/ATP stimulation of cultured primary neuron. Caspase-1 deficiency reduced the caspase-1 activity enhancement. d Caspase-1 deficiency decreased pyroptosis-related protein expression and reduced caspase-1 and its fragments (p10 and p20) leakage. The histogram was used to analyze protein expression in the cell lysate. Data are presented as the mean ± SEM. **p < 0.01 and ***p < 0.001 versus normal group, #p < 0.05, ##p < 0.01, and ###p < 0.001 versus control group, one representative experiment of five was shown