Fig. 1

T. gondii associated with neurons in the brain. a Brains from mock-infected control mice or mice orally infected with 10 ME49 cysts after day 15 post infection were perfused with ice-cold PBS. Cryo-sections were fixed and stained with neurotracer (green) and anti-T. gondii antibody (red). Representative images from cortex and hippocampal areas of the brains of control and T. gondii-infected mice were captured and magnified images of boxed regions are shown on the right (n = 7). This experiment was repeated three times. b Whole brain scans of control and T. gondii-infected mice are shown for days 30 and 60 post infection. Zoomed in images show BAG1⁺ cysts in the cortical regions of the brain. Graph represents BAG1⁺ cyst counts for days 15, 30, 60, and 90 post infection (n = 3–4 mice per group). The experiment was repeated three times. Scale bar = 3 mm. Data are shown as mean ± SEM. *p < 0.05