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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Intraocular DHODH-inhibitor PP-001 suppresses relapsing experimental uveitis and cytokine production of human lymphocytes, but not of RPE cells

Fig. 3

Proliferation and survival (metabolic activity) of PHA-stimulated human PBL and ARPE-19 cells treated with PP-001. a Inhibition of PHA-stimulated human peripheral blood cells from four different donors with PP-001, determined by XTT assay. OD 490 was measured after 72 h of PBL culture in triplicates with medium only, PHA and PHA with different concentrations of PP-001. Mean medium control values were subtracted from the mean values of stimulated cultures of each individual donor. Data of cultures stimulated with PHA only were defined as 100%; the percentage of activity (OD 490) is calculated in relation to the PHA control for each donor. Data show the means of % OD490 + SE from all donors. b XTT assay of triplicate cultures of ARPE-19 cells being confluent for 4 days and treated with either PP-001 or medium only. OD 490 was determined after 72 h of culture. Data are shown as mean OD 490 + SD

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