Fig. 5

Cytokine/chemokine of human RPE cells treated with PP-001 or bevacizumab. Secretion of various factors by ARPE-19 cell line or two different primary human RPE cells (RPE 80.1 and RPE 81.6) cultured with different concentrations of PP-001 (black columns) or bevacizumab (μg/well). Supernatants from triplicate cultures collected at three time points (24, 48, and 72 h after onset of cultures, in total nine samples) were pooled for the final bioplex analysis. Data show the mean fluorescence of > 50 measured beads/analyte. “Control” (white columns) are the supernatants from cells cultured without inhibitor. Dashed lines indicate the baseline secretion of the cells according to the control culture