Fig. 3

hUCB cell types prevent brain tissue loss and cell death following perinatal HI brain injury. a Percentage of left hemisphere volume of the total brain. b Number of TUNEL-positive cells in the frontal cortex of brains. Representative images of cresyl violet- and acid fuschin-stained brain sections used for volumetric analysis for sham (c), HI (d), HI + UCB (e), HI + Tregs (f), HI + EPCs (g), and HI + monocytes (h). Representative images (× 400 magnification) of TUNEL-positive cells (indicated by black arrows) in the cortex of HI (i), HI + UCB (j), and HI + EPCs (k). (n = 5–8 pups per group, *P < 0.05 compared to sham, ^P < 0.05 compared to HI, scale bar = 3 mm)