Fig. 4

Perinatal HI injury increases infiltration of CD4+ cells into the brain, and hUCB cells can prevent this infiltration. Mononuclear cells were isolated from the left hemisphere of pups 7 days after HI injury, and the phenotype of T helper cells was assessed by flow cytometry. a Proportion of CD4+ lymphocytes. b Proportion of CD4+IL4+ T cells. c Proportion of CD4+IFN-γ+ T cells. d Proportion of CD4+IL17+ T cells. e Ratio of Th1 cells (IFN-γ+) to Th2 cells (IL4+). f Correlation between Th1:Th2 vs time to turn in negative geotaxis test. g Ratio of Th17 cells (IL-17+) to Th2 cells (IL4+). (n = 8–13 pups per group, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05 compared to sham. ^^^^P < 0.0001, ^^^P < 0.001, ^^P < 0.01 compared to HI)