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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Differential effect of angiotensin II and blood pressure on hippocampal inflammation in mice

Fig. 5

Effect of Ang II on microglial process ultrastructure. Differences in microglial process ultrastructure between control (CTL) and Ang II 1000 ng/kg/min treated animals, as observed within the hippocampal DG polymorphic layer. The area (A), perimeter (B), circularity (C), and solidity (D) were used to assess changes in morphology. Vacuoles (E) indicative of autophagy or phagocytosis were counted on a microglial process basis. The proportion of microglial processes associated with pockets of extracellular space containing cellular debris showing signs of digestion, termed “extracellular digestion” (F) was also determined (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, by Mann-Whitney test, n = 3–4) a.u.= arbitrary units. G Representative pictures of Iba-1-stained microglial cell bodies and processes captured at a magnification of × 6800. Typical Iba-1-positive microglial process (m) observed in a control animal (upper left). Examples of Iba-1-positive microglial processes in Ang II-treated animals (upper right and lower panels). The microglial process is making direct contacts with a pre-synaptic axon terminal (t), a synaptic cleft (arrowhead), and a post-synaptic dendritic spine (s) (upper right). The elongated microglial process with a complex morphology that contains an accumulation of lipofuscin granules, a hallmark of cellular aging (g) (lower left). The microglial process is associated with a pocket of extracellular space that contains cellular debris undergoing digestion (asterisk). d dendrite (lower right)

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