Fig. 2

Sub-acute Prussian Blue (PB)-positive cerebral microhemorrhages (CMH). Significantly higher number of PB-positive lesions in LPS-treated young (n = 10) and LPS-treated aged mice (n = 15) compared with their respective saline controls (n = 10 per group) (a). Significant increase in PB-positive lesion size with aging (b). Significant increase in PB-positive total lesion area in LPS-treated aged mice compared with LPS-treated young mice and saline-treated aged mice (c). PB-positive and H&E-positive lesion number are significantly correlated in aged mice (d). Representative images showing PB-positive stains in different brain regions (e). Scale bar = 100 μm. Data are presented as mean ± SEM. One-way ANOVA with Bonferroni’s post-test or Kruskal-Wallis test with Dunn’s post-test; *p < 0.05, **p < 0.01, and ***p < 0.001