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Table 1 List of antibodies used for flow cytometric and immunohistochemical assessment of immune cells

From: Neuregulin-1 elicits a regulatory immune response following traumatic spinal cord injury

Antibody Color Application Cat number Concentration
CD3 PerCP Flow eBioscience, 46-00-30-82 1:20
CD4 BV510 Flow, IHC BD, 740138 1:20, (1:100 for IHC)
Ms IgG2a, k BV510 Flow BD, 563027 1:20
CD45 APC-Cy7 Flow BD, 561586 1:20
Ms IgG1, k APC-Cy7 Flow BD, 557873 1:20
IFN-γ FITC Flow BD, 559498 1:20
Ms IgG1, k FITC Flow BD, 554679 1:20
IL-10 PE Flow BD, 555088 1:20
Ms IgG2b, k PE Flow BD, 555058 1:20
FoxP3 APC Flow, IHC eBioscience, 17-5773-80 1:20, (1:100 for IHC)
Ms IgG2a, k APC Flow eBioscience, 17-4724-42 1:20
CD68 FITC Flow, IHC Bio-Rad, MCA341F 1:20, (1:100 for IHC)
Ms IgG1 FITC Flow Bio-Rad, MCA1209F 1:20
CD163 PE Flow, IHC Bio-Rad, MCA342R 1:20, (1:100 for IHC)
Ms IgG1 PE Flow Bio-Rad, MCA1209PE 1:20
CD86 BV-421 Flow, IHC BD, 743,211 1:20, (1:100 for IHC)
Ms IgG1, k BV-421 Flow BD, 562438 1:20
IL-10 Alexa 647 Flow BD, 562156 1:20
Ms IgG2b, k Alexa 647 Flow BD, 557903 1:20
CD45RA APC-Cy7 Flow BD, 561624 1:20
Armenian hamster IgG isotype control PE-Cy7 Flow eBioscience, 25-4888-82 1:20
Anti Iba-1, rabbit Unconjugated IHC Wako, 019-19741 1:500
Anti CD3, rabbit Unconjugated IHC Abcam, GR2952321 1:300
Anti IL-10, mouse Unconjugated IHC R&D, MAB519 1:300
Goat anti-rabbit secondary Ab Alexa Fluor® 647 IHC Invitrogen, A21245 1:400
Goat anti-mouse
secondary Ab
Alexa Fluor® 568 IHC Invitrogen, A11031 1:400