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Table 1 List of antibodies used for flow cytometric and immunohistochemical assessment of immune cells

From: Neuregulin-1 elicits a regulatory immune response following traumatic spinal cord injury

Antibody

Color

Application

Cat number

Concentration

CD3

PerCP

Flow

eBioscience, 46-00-30-82

1:20

CD4

BV510

Flow, IHC

BD, 740138

1:20, (1:100 for IHC)

Ms IgG2a, k

BV510

Flow

BD, 563027

1:20

CD45

APC-Cy7

Flow

BD, 561586

1:20

Ms IgG1, k

APC-Cy7

Flow

BD, 557873

1:20

IFN-γ

FITC

Flow

BD, 559498

1:20

Ms IgG1, k

FITC

Flow

BD, 554679

1:20

IL-10

PE

Flow

BD, 555088

1:20

Ms IgG2b, k

PE

Flow

BD, 555058

1:20

FoxP3

APC

Flow, IHC

eBioscience, 17-5773-80

1:20, (1:100 for IHC)

Ms IgG2a, k

APC

Flow

eBioscience, 17-4724-42

1:20

CD68

FITC

Flow, IHC

Bio-Rad, MCA341F

1:20, (1:100 for IHC)

Ms IgG1

FITC

Flow

Bio-Rad, MCA1209F

1:20

CD163

PE

Flow, IHC

Bio-Rad, MCA342R

1:20, (1:100 for IHC)

Ms IgG1

PE

Flow

Bio-Rad, MCA1209PE

1:20

CD86

BV-421

Flow, IHC

BD, 743,211

1:20, (1:100 for IHC)

Ms IgG1, k

BV-421

Flow

BD, 562438

1:20

IL-10

Alexa 647

Flow

BD, 562156

1:20

Ms IgG2b, k

Alexa 647

Flow

BD, 557903

1:20

CD45RA

APC-Cy7

Flow

BD, 561624

1:20

Armenian hamster IgG isotype control

PE-Cy7

Flow

eBioscience, 25-4888-82

1:20

Anti Iba-1, rabbit

Unconjugated

IHC

Wako, 019-19741

1:500

Anti CD3, rabbit

Unconjugated

IHC

Abcam, GR2952321

1:300

Anti IL-10, mouse

Unconjugated

IHC

R&D, MAB519

1:300

Goat anti-rabbit secondary Ab

Alexa Fluor® 647

IHC

Invitrogen, A21245

1:400

Goat anti-mouse

secondary Ab

Alexa Fluor® 568

IHC

Invitrogen, A11031

1:400