Fig. 1

VCE-004.8 mediates HIF-1α stabilization. a NIH3T3-EPO-Luc cells were stimulated with VCE-004.8 at the doses indicated for 6 h and assayed for luciferase activity. b Cells were pre-treated with VCE-004.8 for 1 h and then washed or not with PBS and incubated in complete medium for 6 h. Fold induction relative to untreated cells is shown. Data represent the mean ± SD (n = 5). ***p < 0.001 VCE-004.8 or DFX-treated cells vs untreated cells (one-way ANOVA followed Tukey’s test). c, d HMC3 and HMEC-1 cells respectively were treated with VCE-04.8 and further analyzed for HIF-1α, HIF-2α, PHD1, PHD2, and PHD3 expression by immunoblot (n = 3)