Fig. 7

VCE-004.8 significantly reduces microglial reactivity and infiltration of inflammatory cells and preserves myelin structure in EAE animals. Cross-sectional images of thoracic–lumbar spinal cord cross-sections of 30 μm thick, in which immunofluorescence with anti-Iba1 (a), H-E (b), cryomyelin (c), and neurofilament H staining (d) immunohistochemistry was performed. Treatment with VCE-004.8 reduces the number of cell infiltrates (b, arrows) and a significant decrease in microglial reactivity (a). The quantifications are shown as means ± SEM, and significance was determined by Kruskal–Wallis non-parametric test. ***p < 0.001 vs CFA; ^#p < 0.05 ^###p < 0.001 vs EAE + VEH. Scale bar: 150 μm (a), 100 μm (c and e). *p < 0.05 unpaired two-tailed Student’s t test (d)