Fig. 2
Chronic exposure to activated monocyte supernatants or TNFα decreases visual cycle gene expression in the RPE. a Representative pictures of RPE staining with OTX2 (Orthodenticle homeobox 2, red) antibody and Arrayscan fluorescence intensity quantification of OTX-2 staining after 10 days of culture with or without lipopolysaccharide-activated monocyte supernatants (Mo SN) or TNFα added daily at different concentrations. b TTR (transthyretin) and c RPE65 (retinal pigment epithelium specific 65 kDa protein)-mRNA expression normalized with S26 expression quantified by RT-qPCR of 10-day RPE culture exposed to the indicated TNFα concentrations (n = 5/group one-way ANOVA or Mann-Whitney, comparison versus control * P < 0.05,**P < 0.005, ***P < 0.0005)