Fig. 3
Chronic exposure to TNFα disrupts RPE barrier properties. a Measurements of RPE transepithelial electric resistance (TER) measured daily in a 10-day trans-well culture with or without different concentrations of TNFα (n = 3/group, one-way ANOVA, versus control for each time point *P < 0.05, **P < 0.005, ***P < 0.0005). b Representative pictures of RPE staining with Zonula occludens(ZO) 1 (green) and F actin (Phalloidin, red). c–e Relative expression of OCLD (Occludin), CLD19 (Claudin-19), and ACTA2 (Smooth muscle alpha (a)-2 actin) mRNA expression normalized with S26 quantified by RT-qPCR of 10-day RPE culture with or without the indicated TNFα concentrations (n = 5/group one-way ANOVA, versus control *P < 0.05, **P < 0.005, ***P < 0.0005)