Fig. 3

Increased infiltration of CD4⁺ T-cells into the DRG of LP-BM5-infected animals. Mononuclear cells from six ganglia (L3-L5) were isolated using a non-enzymatic dissociation method and were labeled with Abs specific for anti-CD45-PE-Cy5, anti-CD4-BV510, and anti-CD8-PE-Cy7 for analysis by flow cytometry. a Representative contour plots show the percentages of CD4⁺ and CD8⁺ T lymphocytes within uninfected and infected (LP-BM5) DRG of WT animals at 10 weeks p.i. b CD4⁺ T-cell infiltration into the DRG of uninfected and LP-BM5-infected animals. c CD8⁺ T-cell infiltration into the DRG of uninfected and infected animals. Pooled data present absolute numbers (mean ± SD) of CD4⁺ and CD8⁺ T-cells from two independent experiments using 4–6 animals per group. *p < 0.05