Fig. 5
From: Nitrosative damage during retrovirus infection-induced neuropathic pain
NO-induced damage within the LSC and DRG of LP-BM5-infected animals. a Western blot of LSC tissue lysates from uninfected (UI, lanes 1 to 4) and LP-BM5-infected (Inf, lanes 5 to 9) animals probed with anti-3-nitrotyrosine antibodies. Each lane displays tissue extract from one animal at 10 weeks p.i. The band labeled a identifies a high molecular weight (MW) protein band, while the b band points out a lower MW protein, both of which are nitrosylated. The signal intensity of both the a and b protein bands for uninfected (UI) and infected (IF) animals was measured using densitometry and plotted. **p < 0.01 (b) Western blot of lysates obtained from uninfected (UI, lane 1) and LP-BM5 infected (Inf, lanes 2 to 5) animals’ DRG (L3-L5) probed with anti-3-nitrotyrosine antibodies. Each lane displays a tissue extract pooled from three individual animals at 10 weeks p.i. The band labeled a represents a high MW protein, and the b band represents the lower MW protein band that are nitrosylated. The signal intensity of both a and b protein bands for uninfected (UI) and infected (IF) animals was measured and is plotted alongside. **p < 0.01 M = protein molecular weight marker ranging from size 15 to 250 kD. c IHC staining of LSC and DRG sections (L3-L5) from uninfected and infected (LP-BM5) mice with anti-3-nitrotyrosine antibody (brown) and mouse IgG isotype antibody. Scale bar = 100 μm for LSC and 50 μm for DRG