Fig. 7

Accelerated onset of hind-paw mechanical hypersensitivity in PD-1 KO animals infected with LP-BM5 and associated cellular infiltration. Hind-paw withdrawal threshold was assessed in both WT, as well as PD-1 KO, animals as described in Fig. 1. a Paw withdrawal threshold (in grams) was assessed at various time points in LP-BM5-infected (Inf) PD-1 KO animals, as well their age-matched uninfected (UI) littermates (n = 10/group). *p < 0.05 between infected (Inf) and uninfected (UI) PD-1 KO animals, and #p < 0.05 between PD-1KO-infected (Inf) and uninfected (UI) PD-1 KO animals (left and right paws, respectively). b Paw withdrawal threshold was assessed at various time points in LP-BM5-infected WT, as well as PD-1 KO animals (n = 10/group). *p < 0.05 between infected PD-1 KO and infected WT animals, and #p < 0.05 between infected PD-1 KO and infected WT animals (left and right paws, respectively). Data are presented as mean ± SEM paw withdrawal threshold. c Representative contour plots show the percentages of CD4⁺ and CD8⁺ T lymphocytes within infected spinal cords of WT, as well as PD-1 KO animals at 4 weeks p.i. d CD4⁺ T-cell infiltration into the spinal cord of uninfected and infected WT and PD-1 KO animals. e CD8⁺ T-cell infiltration into the spinal cord of uninfected and infected WT and PD-1 KO animals. Pooled data present absolute numbers (mean ± SD) of CD4⁺ and CD8⁺ T-cells from two independent experiments using three animals per group. **p < 0.01