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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Early alpha-lipoic acid therapy protects from degeneration of the inner retinal layers and vision loss in an experimental autoimmune encephalomyelitis-optic neuritis model

Fig. 6

Microglial activation and T cell infiltration were not altered in optic nerves of EAE mice after LA treatment. LA also failed to reduce demyelinating lesions. One hundred twenty days after MOG immunization, mice were sacrificed and optic nerves were extracted and processed, and longitudinal sections were prepared for Iba1, MBP, and CD3 staining (a). Optic nerves of sham-EAE, MOG EAE, and MOG EAE with LA-treated mice were compared for microglia activation by fluorescence intensity measurement, by an MBP myelination score for myelin status, and by a CD3 score for T cell Infiltration; quantitative analyses of the results of three independent EAE experiments with at least four mice are shown as bar graphs; bar = 50 μm (b); one optic nerve per mouse was included. (***p < 0.001, n.s. = not significant, by ANOVA with Dunnett’s post hoc test compared to MOG-untreated mice)

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