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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Oxysterol levels and metabolism in the course of neuroinflammation: insights from in vitro and in vivo models

Fig. 3

Kinetic of mRNA expression of oxysterol-metabolizing enzymes in LPS-activated BV2 cells compared to control cells. 2.5 × 105 cells were incubated with LPS (100 ng/mL) or vehicle (CTL) for the indicated time points. mRNA was extracted and RT-qPCR performed for CYP27A1, cholesterol 25 hydroxylase (Chol25OHase), HSD3B7, 11β-HSD1, and the catalytic subunit of cholesterol epoxyde hydrolase (D8D7l). The data are expressed as the mean ± SEM in percentage of their respective CTL. ****p < 0.0001; ***p < 0.001; **p < 0.01; and *p < 0.05 for LPS-activated cells versus their respective controls. The different letters (a, b, c, d) indicate differences between the time points

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