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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Oxysterol levels and metabolism in the course of neuroinflammation: insights from in vitro and in vivo models

Fig. 5

Effect of LPS activation of primary co-culture of glial cells on oxysterol levels. The cells were incubated in media with 1% FBS and containing 100 ng/mL of lipopolysaccharide (LPS) or vehicle (CTL). Oxysterols were analyzed at 8 and 24 h. a Oxysterol levels in LPS-activated primary co-culture of glial cells in comparison to control cells. The data are expressed as the mean ± SEM in percentage of their respective controls (CTL). ****p < 0.0001; ***p < 0.001; and **p < 0.01 for LPS-activated cells versus their respective controls. The different letters (a, b) indicate differences between the time points. b, c Multivariate analysis of the variations in oxysterol levels. The results of the partial least-squares discriminant analysis (PLS-DA) are shown as score plots for the two time points analyzed: b 8 and c 24 h. Each dot on the graph represents a dish of cells. The quality of the model was assessed by the values of R2 and Q2. These values were 0.94 and 0.80, respectively, after 8 h and 0.95 and 0.91 after 24 h

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