Fig. 6
mRNA expression of oxysterol-metabolizing enzymes in LPS-activated co-culture of primary glial cells. 2.5 × 105 cells were incubated with 100 ng/mL of LPS or vehicle (CTL) for 8 h. mRNA was extracted and RT-qPCR performed for CYP27A1, cholesterol 25 hydroxylase (Chol25OHase), HSD3B7, CYP7B1, and the catalytic subunit of cholesterol epoxyde hydrolase (D8D7l). The data are expressed as the mean ± SEM in percentage of their respective CTL. ****p < 0.0001 and ***p < 0.001 for LPS-activated cells versus CTL