Fig. 7
Oxysterols’ effects on mRNA expression of pro-inflammatory markers in LPS-activated co-culture of primary glial cells. 2.5 × 105 cells were incubated with 10 μM of each oxysterol or vehicle (DMSO, Veh.). LPS (100 ng/mL) was added an hour later for 8 h. mRNA was extracted, and RT-qPCR performed. The data are expressed as the mean ± SEM in percentage of vehicle (Veh.) ****p < 0.0001; ***p < 0.001; **p < 0.01; and *p < 0.05 vs Veh