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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activation of the NF-κB signaling pathway in cerebral ischemia/reperfusion rats

Fig. 5

OTULIN was markedly increased in activated microglial cells. a Analysis of immunofluorescent confocal images showed that OTULIN was increased in activated microglial cells after ischemic stroke (OTULIN, red; Iba-1, green; DAPI, blue; scale bar = 75 μm; n = 6). The red boxes indicate enlarged areas. The levels of OTULIN mRNA (b, n = 6) and protein (c, d; n = 3) in PM and N9 cells were detected at 24 h after exposure to regular microglia medium, conditioned medium (CM) collected from OGD-treated neuronal cultures (OGD[+]CM), or OGD-untreated (OGD[−])CM. All values are presented as the means ± SEMs; ###P < 0.001 versus the NC group; &&&P < 0.001, &&P < 0.01 versus the OGD(−)CM group

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Journal of Neuroinflammation

ISSN: 1742-2094