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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activation of the NF-κB signaling pathway in cerebral ischemia/reperfusion rats

Fig. 7

Overexpression of OTULIN suppressed the levels of the pro-inflammatory cytokines produced by activated PM and N9 cells through depressing the NF-κB pathway. ELISA was used to measure the levels of TNF-α (a; n = 6), IL-1β (b; n = 6), and IL-6 (c; n = 6) in the NC, OGD(−)CM, and OGD(+)CM groups of PM and N9 cells. Each group was transfected with the LV-OTULIN or LV-Scramble. OTULIN and NF-κB pathway-associated proteins including p-IκBα, IκBα, and cytoplasm/nucleus NF-κB p65 in PM (d, e; n = 3) and N9 (d, f; n = 3) cells were detected by Western blot and analyzed quantitatively. All values are presented as the means ± SEMs. ***P < 0.001, **P < 0.01, and *P < 0.05 versus the OGD(−)+LV-OTULIN group; &&&P < 0.001, &&P < 0.01, and &P < 0.05 versus the OGD(+)+LV-Scramble group

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Journal of Neuroinflammation

ISSN: 1742-2094