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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarachnoid hemorrhage

Fig. 2

The microglia were activated by Prx2 and polarized to M1-like microglia. a The mRNA level of IL-1β, IL-6, and TNF-α in microglia increased dramatically after SAH. The IL-1β and IL-6 mRNA level in OxyHb + Prx2 10 μg/mL group increased significantly after Prx2 treatment when compared with OxyHb group. The mRNA level of TNF-α remained unchanged. b The concentrations of IL-1β, IL-6, and TNF-α in culture medium of microglia were determined by ELISA. Consistent with the mRNA level, the IL-1β and IL-6 concentrations in OxyHb + Prx2 10 μg/mL group increased significantly after Prx2 treatment compared with the OxyHb group. However, the concentration of TNF-α remained unchanged. c The phase-contrast photomicrographs of cultured microglia. Ramified microglia in the control group became less branching after SAH. Incubated with Prx2, the number of microglial branches became more less. Scale bar = 50 μm. ##P < 0.01, ###P < 0.001 vs control; **P < 0.01, ***P < 0.001, nsP > 0.05 vs indicated groups. Bars represent the mean ± SD. N = 6 in each group

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