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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Glutaminase 1 regulates the release of extracellular vesicles during neuroinflammation through key metabolic intermediate alpha-ketoglutarate

Fig. 8

Brain-specific GLS1 overexpression increases EV release in vivo. a Wild-type and Nestin-GAC mice were sacrificed at 12–16 weeks old. The brains were removed, and for each brain, the right hemibrain was processed for EVs isolation and the left hemibrain was homogenized for brain lysates. Protein lysates were prepared from the EVs pellets in wild-type and Nestin-GAC hemibrains. The levels of EVs markers, tTG and flotillin-2, in the EVs lysates were analyzed by Western blot. EV protein loading was normalized with the weight of their corresponding hemibrains before the EV isolation. b, c Densitometric quantifications of the protein levels were presented as fold changes relative to that in wild-type EV lysate. Unpaired t test, n = 4 per genotype. Error bars are means ± S.E.M. df The brain tissues from adult Thy1-GAC mice were dissected, and EVs were isolated using the same techniques described above. EVs collected from WT (d) and Thy1-GAC (e) were evaluated via negative staining under TEM. Images shown were representative of each group. EV numbers per vision fields (n = 10) were quantified. CTL, control. Unpaired t test, *** denotes p < 0.001

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