Fig. 4

IL-33/ST2 signaling in microglia is essential for celastrol treatment-induced neuroprotective effects in vitro. Neuron-microglia co-cultures or neuron only cultures with or without OGD for 3 h were treated with a range of IL-33 concentrations, celastrol, or PBS (control), respectively, for 24 h. a Neuronal activity in IL-33-treated neuron-microglia co-cultures was detected using CCK8. The data are presented as mean ± SD. ^**P < 0.01 vs. control group. b Neuronal activity in IL-33-treated neuron exposed to OGD for 3 h was detected using CCK8. c Neuronal activity in celastrol-treated neuron-microglia co-cultures was detected using CCK8. The data are presented as mean ± SD. ^*P < 0.05, ^**P < 0.01 vs. control group. d Neuronal activity in celastrol-treated neuron exposed to OGD for 3 h was detected using CCK8. Each experiment repeated 6 times