Fig. 5

ST2 signaling is essential for M2 polarization after ischemia. Microglial cells transfected with or without siRNA against ST2 were exposed to OGD conditions for 3 h before treatment with either IL-33 (50 ng/mL), celastrol (1 μM), or PBS (control) for 24 h. Western blot (a) and qRT-PCR (b) were used to detect the expression of ST2 after transfection with siRNA against ST2. The data are presented as mean ± SD. ^***P < 0.001 vs. control group. c Representative images of double immunofluorescent staining of microglia with the microglia/macrophage marker, Iba1 (red), and the M1 marker, CD16 (green). Scale bar = 50 μm. d Quantification of the percentage of CD16⁺/Iba-1⁺ cells among total Iba-1⁺ cells. The data are presented as mean ± SD. ^***P < 0.001 vs. control group. ^###P < 0.001 vs. OGD group. e Representative images of double immunofluorescent staining of microglia with the microglia/macrophage marker, Iba1 (red), and the M1 marker, CD16 (green), after transfection with siRNA against ST2. Scale bar = 50 μm. f Quantification of the percentage of CD16⁺/Iba-1⁺ cells among total Iba-1⁺ cells. The data are presented as mean ± SD. ^***P < 0.001 vs. control group. g Representative images of double immunofluorescent staining of microglia with the microglia/macrophage marker, Iba1, and the M2 marker, CD206. Scale bar = 50 μm. h Quantification of the percentage of CD206⁺/Iba-1⁺ cells among total Iba-1⁺ cells. The data are presented as mean ± SD. ^***P < 0.001 vs. control group. ^#P < 0.05 vs. OGD group. i Representative images of double immunofluorescent staining of the microglia/macrophage marker, Iba1, and the M2 marker, CD206, after transfection with siRNA against ST2. Scale bar = 50 μm. j Quantification of the percentage of CD206⁺/Iba-1⁺ cells among total Iba-1⁺ cells. The data are presented as mean ± SD. ^***P < 0.001 vs. control group. Each experiment repeated 6 times