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Fig. 11 | Journal of Neuroinflammation

Fig. 11

From: Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury

Fig. 11

Blocking LAR and PTPσ receptors has no effect on formation of astrocytic scar and CSPGs following SCI. a To study scar formation under ILP and ISP treatment, we examined GFAP protein expression by Western blotting at 1, 3, 5, 7, and 14 days post-injury. As anticipated, GFAP protein expression was significantly increased following injury compared to uninjured animals. However, ILP and ISP treatment had no significant effect on the expression of GFAP at any time-points after SCI. b Using immunohistochemistry, we also studied astrocytic scar in SCI lesion. Our quantitative immunodensity analysis in the injured spinal cord tissue at the chronic 28 days post-SCI also showed no significant differences in the levels of GFAP under ILP/ISP treatment compared to vehicle SCI control group. c Slot blot analysis of CS56 expression at 1, 3, 5, 7, and 14 days post-SCI demonstrated a significant increase CSPGs following injury. Similar to GFAP, ILP and ISP treatment had no effect on the deposition of CSPGs following injury. d CS56 expression was additionally measured using immunohistochemistry at different distances to injury epicenter at 28 days post-SCI. ILP/ISP treatment showed no significant differences in the levels of CS56 compared to vehicle SCI control group. The data show the mean ± SEM, *p < 0.05, **p < 0.001, one-way ANOVA (a, c), two-way ANOVA (bd), N = 4–6/group

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