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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury

Fig. 3

Inhibition of LAR and PTPσ promotes an increase in the subpopulation of M2 macrophages after SCI. a Western blot analysis of Iba1 protein expression at 1, 3, 5, 7, and 14 days following SCI revealed no apparent change in the presence of microglia/macrophage within SCI lesion. b, c Similarly, flow cytometric analysis of spinal cord tissue revealed no change in the total number of infiltrated macrophages (CD45+/CD68+) at 3 and 7 days post-injury between vehicle control and ILP/ISP (10 μg/day) treated SCI animals. d, e ILP/ISP treatment resulted in a non-significant decrease in the number of CD45+/CD68+/CD86+ M1 macrophages at 3 and 7 days post-injury. f, g A significant increase in the number of CD45+/CD68+CD163+ M2 macrophages was observed at 7 days post-injury in ILP/ISP-treated animals. h Representative flow cytometry gates are depicted. N = 4–6 animals/group/time-point. The data show mean ± SEM, *p < 0.05, one-way ANOVA (a), Student t test (bg)

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