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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury

Fig. 9

IL-10 promotes oligodendrocyte differentiation of spinal cord NPCs in vitro. a–c Addition of recombinant IL-10 had no apparent effect on NPCs proliferation (BrdU+/DAPI+) at all doses tested. d However, IL-10 significantly reduced the percentage of GFAP+/DAPI+ astrocytes at 100 and 200 ng/ml, e while increased the percentage of Olig2+ cells at 50, 100, 200, and 400 ng/ml with the highest effect at 200 ng. This effect was significantly attenuated with IL-10 neutralizing antibody. Addition of 0.8 μg/ml of IL10 neutralizing antibody effectively blocked 100 ng/ml of IL-10 on astrocyte and oligodendrocyte differentiation of NPCs. fk Representative images of NPC differentiation assessment are shown. N = 3–5 independent experiments. The data show the mean ± SEM, *p < 0.05, one-way ANOVA

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