Table 2 List of flow cytometry antibodies used in this study
Antibody | Color | Company-Cat number | Dilution factor |
|---|---|---|---|
CD3 | PerCP | eBioscience, 46-00-30-82 | 1:20 |
CD4 | BV510 | BD, 740138 | 1:20 |
Ms IgG2a, k | BV510 | BD, 563027 | 1:20 |
CD45 | APC-Cy7 | BD, 561586 | 1:20 |
Ms IgG1, k | APC-Cy7 | BD, 557873 | 1:20 |
IFN-γ | FITC | BD, 559498 | 1:20 |
Ms IgG1, k | FITC | BD, 554679 | 1:20 |
IL-10 | PE | BD, 555088 | 1:20 |
Ms IgG2b, k | PE | BD, 555058 | 1:20 |
FoxP3 | APC | eBioscience, 17-5773-80 | 1:20 |
Ms IgG2a, k | APC | eBioscience, 17-4724-42 | 1:20 |
CD68 | FITC | Bio-Rad, MCA341F | 1:20 |
Ms IgG1 | FITC | Bio-Rad, MCA1209F | 1:20 |
CD163 | PE | Bio-Rad, MCA342R | 1:20 |
Ms IgG1 | PE | Bio-Rad, MCA1209PE | 1:20 |
CD86 | BV-421 | BD, 743211 | 1:20 |
Ms IgG1, k | BV-421 | BD, 562438 | 1:20 |
IL-10 | Alexa 647 | BD, 562156 | 1:20 |
Ms IgG2b, k | Alexa 647 | BD, 557903 | 1:20 |
TNFα | PE-Cy7 | eBiosicence, 25-7423 | 1:20 |
Armenian Hamster IgG isotype control | PE-Cy7 | eBioscience, 25-4888-82 | 1:20 |