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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Inhibition of peripheral macrophages by nicotinic acetylcholine receptor agonists suppresses spinal microglial activation and neuropathic pain in mice with peripheral nerve injury

Fig. 2

Inhibition of interleukin-1β (IL-1β) overexpression in macrophages by TC-2559. Mice were subject to PSL or sham surgery. TC-2559 was administered subcutaneously (s.c., 22.8 μmol/kg) or perineurally (p.n., 20 nmol) on days 0, 1, 2, and 3, and the SCN was collected. Expression levels of IL-1β mRNA were analyzed by RT-qPCR. a Time course of IL-1β mRNA expression in the SCN on days 1 to 14 after sham or PSL. Inhibition of PSL-induced upregulation of IL-1β mRNA in the SCN on day 7 after PSL by s.c. (b) or p.n. (c) administration of TC-2559. d Peritoneal macrophages collected from naïve mice were treated with lipopolysaccharide (LPS, 50 ng/ml) and TC-2559 (0.5 mM), and cells were incubated for 24 h. Inhibition of LPS-induced upregulation of IL-1β mRNA by TC-2559 is shown. Data are presented as the mean ± SEM; n = 5–12. ***P < 0.001 versus sham. ###P < 0.001, #P < 0.05 versus PSL/Veh or LPS/Veh

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Journal of Neuroinflammation

ISSN: 1742-2094