Table 1 Primer sequences for RT-qPCR
Gene | Forward (5′ to 3′) | Reverse (5′ to 3′) |
|---|---|---|
GAPDH | GGGTGTGAACCACGAGAAAT | ACTGTGGTCATGAGCCCTTC |
ACTB | CAGCTGAGAGGGAAATCGTG | TCTCCAGGGAGGAAGAGGAT |
IL-1β | AAAGCTCTCCACCTCAATGG | AGGCCACAGGTATTTTGTCG |
Iba1 | ATGAGCCAAAGCAGGGATTT | TTGGGATCATCGAGGAATTG |
CD11b | GTTTCTACTGTCCCCCAGCA | GTTGGAGCCGAACAAATAGC |
CD68 | ACTCATAACCCTGCCACCAC | CCAACAGTGGAGGATCTTGG |
IRF5 | ACACTGAAGGGGTGGATGAG | CGAGGGCCATCATAGAACAG |
IRF7 | GTGTGTCCCCAGGATCATTT | CTGCAGAACCTGAAGCAAGA |
CCL3 | CTGCCCTTGCTGTTCTTCTC | GTGGAATCTTCCGGCTGTAG |