Fig. 3

mAb158 reduces intracellular Aβ load, at least partly through an Fcγ receptor-independent pathway. Aβ₄₂ protofibril accumulation in astrocytes (a) is reduced by preincubating Aβ₄₂ protofibrils with mAb158 (b) or with N297D (c). Although, there was a clear reduction in Aβ accumulation with the presence of N297D as compared to Aβ₄₂ protofibril exposed co-cultures, the cultures treated with mAb158 had significantly lower 555-intensity/number of living cells (d), 555-intensity/number of Aβ inclusions (e), and total 555-stained area of Aβ inclusions (f), compared to cultures treated with N297D. GFAP (green), DAPI (blue), and Aβ (red). Scale bar 20 μm. The experiments were performed in triplicates with independent cell cultures, and 10 images/experiment were analyzed. Statistical analysis using Mann–Whitney U test (***P < 0.001)