Fig. 1

Alterations in chemokine receptor expression in peripheral blood samples. a Heat maps displaying the surface expression of selected inflammatory molecules measured by multicolor flow cytometry on ALS patient or healthy donor (HD)-derived B cells, T cells, monocytes, and NK cells, respectively. Low mean fluorescence intensity (MFI) values were indicated by blue color while high values are displayed in red. Heat maps were generated using the genepattern tool “HeatmapViewer” (Broad Institute, free ware tool). Statistics: unpaired t test. b Surface expression (MFI) of CXCR4, CCR2, CCR5, and CXCR3 on CD3+ T cells of ALS patients and healthy donors measured by flow cytometry. Statistical analysis was performed using unpaired t test (n = 10; *p < 0.05,). c MFI of CCR2 and CD11B on CD14 + CD16 classical monocytes and CD14+ CD16+ non classical monocytes of ALS patients and controls measured by flow cytometry (unpaired t test n = 10). d Aggressiveness of diseases calculated as D50 (period of time in months until the ALSFRS-R decreases to 24 points/50%)) correlated with the MFI of CXCR3 on the T cells from ALS patients measured by flow cytometry (spearman correlation, one-tailed test)