Fig. 1

Expression profile of MC4R and p-AMPK after ICH. a Representative western blot bands of time course and quantitative analyses of MC4R expression in the ipsilateral hemisphere after ICH. b Representative western blot bands of time course and quantitative analyses of phosphorylated AMPK expression in the ipsilateral hemisphere after ICH. *p < 0.05 vs sham. Error bars are represented as mean ± SD. One-way ANOVA, Tukey’s test, n = 6 per group. c Brain sample with schematic illustration showing the four areas in the perihematomal region (indicated by black squares) from where the images were taken for immunofluorescence staining (pictures are shown in panel d). d Representative images of co-localization of MC4R (red) with microglia/macrophage (Iba-1, green), neurons (NeuN, green), and astrocytes (GFAP, green) in the perihematomal area at 24 h after ICH. Nuclei were stained with DAPI (blue). Scale bar = 50 μm, n = 2