Fig. 3

The effects of RO27-3225 on microglia/macrophage activation and neutrophil infiltration after ICH. a Representative images of immunofluorescence staining of Iba-1 (green) and MPO (green) in the perihematomal area at 24 h after ICH. Brain sample with schematic illustration shows the four areas (indicated by black squares) used for Iba-1 and MPO-positive cell counting in the perihematomal region. b, c Quantitative analyses of Iba-1 and MPO-positive cells in the perihematomal area at 24 h after ICH. d, e Representative western blot bands and quantitative analyses of Iba-1 and MPO protein levels in the ipsilateral hemisphere at 24 h after ICH. *p < 0.05 vs. sham, ^#p < 0.05 vs. ICH + vehicle. Error bars are represented as mean ± SD. One-way ANOVA, Tukey’s test, n = 6 per group