Fig. 6

Mi-sup but not LPS decreased the viability and activated NF-κB p65 of HT-22 cells. MTT assays were performed to detect the cell viability in HT-22 cells treated with LPS (a) or Mi-sup (b) for 12, 24, or 48 h. Results are expressed relative to control and are presented as means ± SEM of three independent experiments (each performed in triplicate). The levels of NF-κB p65 in LPS-exposed BV-2 cells (c) and LPS-exposed HT-22 cells (d) were determined by western blot. HT-22 cells were exposed to Mi-sup for 24 h, and then the activation of NF-κB p65 was determined by western blot (e) and immunofluorescence staining (f). Data are presented as means ± SEM (n = 5). ^*P < 0.05, ^**P < 0.01,^***P < 0.001 vs control group