Fig. 7

Effect of deficiency of ARRB2 on adrenergic-mediated alteration of NF-κB signaling activity and cytokine expression detected at gene level. LPS induced high gene expression of IL-1β (a), IL-8 (b), IL-6 (c), and TNF-α (d). Adrenergic-stimulation considerably declined LPS-induced cytokine gene expression (a–d). Moreover, IL-10 gene expression was significantly increased by adrenergic-stimulation (e). However, deficiency of ARRB2 significantly increased the gene expression of IL-1β (a), IL-8 (b), IL-6 (c), and TNF-α (d), and decreased IL-10 gene expression in spite of adrenergic stimulation. On the other hand, the gene expression of ARRB2 was significantly descended with the transfection of LV3-ARRB2 (h). The gene expression of NF-κB and IκBα was not affected by siRNA transfection (f–g). Data represents each mRNA level relative to β-actin. Each experiment was independently replicated three times. *P < 0.05 and **P < 0.01 by one-way ANOVA