Table 1 All gene primer sequences (Generay Biotech Co., Ltd., Shanghai, China) applied in the qPCR analysis
From: β-arrestin2 functions as a key regulator in the sympathetic-triggered immunodepression after stroke
Gene (GenBank) | Primer sequence (5′-3′) | |
|---|---|---|
IL-6 (NM_000600.3) | Forward | CAGACAGCCACTCACCTC |
Reverse | CTCAAACTCCAAAAGACCAG | |
IL-10 (NM_000572.2) | Forward | GGAGAACCTGAAGACCCT |
Reverse | TGATGAAGATGTCAAACTCACT | |
IL-1β (NM_000576.2) | Forward | ACCACCACTACAGCAAGG |
Reverse | AAAGATGAAGGGAAAGAAGG | |
IL-8 (NM_000584.3) | Forward | GCATAAAGACATACTCCAAACC |
Reverse | AAACTTCTCCACAACCCTCT | |
TNF-α (NM_000594.3) | Forward | TGTAGCAAACCCTCAAGC |
Reverse | GGACCTGGGAGTAGATGAG | |
NF-κB (NM_001165412.1) | Forward | CCACAAGCAAGAAGCTGAAG |
Reverse | AGATACTATCTGTAAGTGAACC | |
IκBα (NM_020529.2) | Forward | ACACTAGAAAACTTCAGATGC |
Reverse | ACACAGTCATCATAGGGCAG | |
ARRB2 (NM_001257331.1) | Forward | TGTGGACACCAACCTCATTG |
Reverse | TCATAGTCGTCATCCTTCATC | |
β-actin (NM_001101.3) | Forward | GCACCACACCTTCTACAATGAG |
Reverse | ATAGCACAGCCTGGATAGCAAC |