Fig. 10

Epidermal Langerhans cell (LC)-deficient fracture mice (Langerhans KO-FX) developed von Frey mechanical allodynia (a) and hindlimb unweighting (b) that did not differ from nociceptive changes observed in wildtype fracture mice (WT-FX) at 3 and 7 weeks after fracture. At the end of the behavioral testing, hind paw skin from WT-FX and Langerhans cell KO-FX mice were evaluated by immunohistochemistry. Langerin/CD-207 (red) was detected in frozen sections from WT-FX mice (c), but not in sections from Langerhans cell KO-FX mice (d). Dotted line indicates the epidermal-dermal boundary. Data were analyzed using a two-way analysis of variance (ANOVA), data are expressed as mean values ± SEM, n = 8–12 per cohort