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Fig. 11 | Journal of Neuroinflammation

Fig. 11

From: Neuropeptide regulation of adaptive immunity in the tibia fracture model of complex regional pain syndrome

Fig. 11

Neuropeptide signaling regulates the post fracture increase in cytokeratin 16 (Krt16) autoantigen expression in fracture limb skin. Western blot analysis demonstrated increased hind paw skin Krt16 levels at 3 weeks after fracture in wildtype fracture mice (WT-FX), vs no fracture controls (WT-NO FX). This increase was blocked by LY303870 treatment and in mice deficient in SP expression (Tac1−/−) after fracture. And Krt16 was found low levels in CGRP receptor signaling-deficient fracture mice (RAMP1−/−-FX). Krt16/actin band intensity ratio was calculated to compare expression levels between the groups. Data were analyzed using a one-way analysis of variance (ANOVA) with Bonferroni correction test for post hoc contrasts, data are expressed as mean values ± SEM, n = 4 per cohort. *** P < 0.001 vs. WT-NO FX, ### P < 0.001 vs. WT-FX

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