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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Neuropeptide regulation of adaptive immunity in the tibia fracture model of complex regional pain syndrome

Fig. 5

Substance P signaling perpetuates the post fracture increase in Langerhans cell (LC) numbers observe in the injured hindlimb skin. Fluorescent immunohistochemical staining of langerin, a LC marker (green), in hind paw skin sections from the control wildtype no fracture mice (a, WT-NO FX) and from the ipsilateral (b, WT-FX-IPSI) and contralateral (c, WT-FX-CONTRA) hind paws of fracture mice at 3 weeks post-injury. After fracture, the epidermal LC numbers increased and cell morphology was altered, with the cells growing larger with increased dendritic branching, compared to the control or the contralateral side. Some LCs appeared to be migrating through the epidermal basement membrane (dashed lines). Daily treatment for 7 days (30 mg/kg/day) with the SP tachykinin1 receptor antagonist LY303870 (d, WT-FX + LY) completely reversed the fracture induced increase in LC numbers. Scale bar = 30 μm. e Quantification of hind paw skin Langerhans cells in tissue sections from no fracture controls, fracture ipsilateral and contralateral hind paws, and fracture mice treated with LY303870. There was a twofold ipsilateral increase in hind paw skin LC numbers after fracture, as compared to the controls, and LY303870 treatment blocked this increase. Data were analyzed using a one-way analysis of variance (ANOVA) with Bonferroni correction test for post hoc contrasts, error bars indicate SEM, n = 6 per cohort. **P < 0.01 for vs. WT-NO FX, ##P < 0.01, ###P < 0.001 vs. WT-FX-IPSI

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