Fig. 11

The effect of WP-sensitization on Iba1 immunoreactive microglia in the old mouse brains. A Microglia were identified using anti-Iba1 immunohistochemical staining in the brain sections (40 μm) of the old sham and WP-sensitized mice. Subtle differences between sham and WP-sensitization in Iba1 immunoreactivity were noted in the hippocampal region of the old mice. (top panels) Representative photomicrographs were taken using a ×4 objective (scale bar = 1 mm). The rectangles within indicates where the high-power photomicrographs a–f were taken using a × 40 objective. B Immunoreactivity to Iba1 (Iba1-IR) within the young and old mouse brains was quantified by densitometric analyses of the digital photomicrographs taken with a ×4 objective. The values indicate group average optical density ± standard error (n = 5–6), *p < 0.05. Top graph, young mice; bottom graph, old mice