Fig. 5

Histological evaluation of the ileal tissues from sham and WP-sensitized young and old mice. Paraformaldehyde-fixed frozen ileal tissues adjacent to Peyer’s patches (labeled with a ‘P’) were cryosectioned at 10 μm. Mast cells were detected as metachromatically stained dark purple cells with TB staining (A) or light purple cells in CMA1 immunohistochemical staining (B). The location and number of the stained cells were qualitatively assessed in sham (a, c, e, g) and WP-sensitized (b, d, f, h) mice. Representative images were taken using a ×10 objective (A, scale bar = 100 μm), or a ×20 objective (B, scale bar = 50 μm)