Fig. 6

Phosphorylated ErbB4 (pErbB4) levels decrease in ECM mice. a Total mRNA level of ErbB4 in brain cortex was unchanged during infection with PbA. b Protein extracts from the cortex and hippocampus of mice were probed with antibodies against phosphorylated ErbB4 (Tyr1284) and total ErbB4. The data represent one of three independent experiments with similar results. Beneath the Western blot is a densitometric analysis of bands assessed with the ImageQuant program. The data are expressed as fold stimulation relative to appropriate controls and are the mean ± SE of duplicated samples performed in three independent experiments. Total protein level of ErbB4 in the brain cortex was unchanged during ECM, whereas phosphorylated ErbB4 (pErbB4) gradually decreased from day 2 and was hardly detectable at day 12. Expression pattern of pErbB4 in hippocampus was similar to that observed in cortex (*p < 0.05, **p < 0.01, compared to uninfected (UN) sample at D2). c Immunofluorescence (IFC) results confirmed the Western blot data, in which total ErbB4 protein was unchanged even 12 days after infection (panels a, b). d IFC data showed that ErbB4 co-localized with neuronal marker-NeuN (panels a–c), but not with microglial cell marker-Iba1 (panels d–f), or astrocyte marker-GFAP (panels g–i)